6-CARBOXYFLUORESCEIN SE
6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER
CAS: 92557-81-8
Molecular Formula: C25H15NO9
6-CARBOXYFLUORESCEIN SE - Names and Identifiers
| Name | 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER |
| Synonyms | 6-FAM-NHS 6-FAM, SE 6-CARBOXYFLUORESCEIN SE 6-CARBOXYFLUORESCEIN-NHS 6-CARBOXYFLUORESCEIN SUCCINIMIDE ESTER 6-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER 6-CARBOXYFLUORESCEIN N-HYDROXYSUCCINIMIDE 6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER 6-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER 6-CARBOXYFLUORESCEIN, SUCCINIMIDYL*ESTER (SINGLE IS 6-carboxyfluorescein, succinimidyl ester (6-FAM, SE) 4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid 1-{[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-6-yl)carbonyl]oxy}pyrrolidine-2,5-dione |
| CAS | 92557-81-8 |
| InChI | InChI=1/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32) |
6-CARBOXYFLUORESCEIN SE - Physico-chemical Properties
| Molecular Formula | C25H15NO9 |
| Molar Mass | 473.39 |
| Density | 1.74±0.1 g/cm3(Predicted) |
| Melting Point | >168°C (dec.) |
| Boling Point | 788.1±70.0 °C(Predicted) |
| Flash Point | 445°C |
| Solubility | Soluble in N,N-dimethylformamide,dimethyl sulfoxide |
| Vapor Presure | 7E-28mmHg at 25°C |
| Appearance | Solid |
| Color | Orange solid |
| Maximum wavelength(λmax) | ['496 nm (Buffer pH9)'] |
| pKa | 9.31 ± 0.20, most acidic, temperature:25 °C |
| Storage Condition | −20°C |
| Stability | Moisture Sensitive |
| Refractive Index | 1.76 |
| MDL | MFCD00077323 |
| Physical and Chemical Properties | 6-carboxyfluorescein succinimide ester is a chemical substance with the molecular formula of C25H15NO9. Fluorescent labeling dyes play an increasingly important role in biological analysis, especially 6-carboxyfluorescein has become an international fluorescent dye for detecting protein and DNA sequence. |
| Use | An amine-reactive succinimidyl ester carboxyfluorescein that yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. |
6-CARBOXYFLUORESCEIN SE - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 8-10-21 |
| HS Code | 29252900 |
6-CARBOXYFLUORESCEIN SE - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 2.112 ml | 10.562 ml | 21.124 ml |
| 5 mM | 0.422 ml | 2.112 ml | 4.225 ml |
| 10 mM | 0.211 ml | 1.056 ml | 2.112 ml |
| 5 mM | 0.042 ml | 0.211 ml | 0.422 ml |
Last Update：2024-01-02 23:10:35
6-CARBOXYFLUORESCEIN SE - Reference Information
| Application in biological field | analysiingaminogcosides; analyzing/determining gabapentin;detecting/analyzing baclofen in human plasma micromolecular level;determining phosphorus-containingamino acids; multiplex genetic analysis;visualizing/detecting proteins; for synthesizingvan MYCIN analoes, vasopressin analoes; as a substrate for measuring kinase activity, phonic activity. |
| biological activity | 6-FAM SE is an isomer of carboxyfluorescein. 6-FAM SE is primarily used for nucleic acid sequencing and labeling of nucleotides. |
| Use | an amine-reactive succinimidyl ester carboxyhalothane, the resulting carboxamide is more resistant to hydrolysis than the fluorescein conjugate prepared with FITC. |
Last Update：2024-04-09 19:05:15
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